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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Defined host factors support HBV infection in non‐hepatic 293T cells
doi: 10.1111/jcmm.14944
Figure Lengend Snippet: Nuclear hormone receptors activate HBV replication in 293T cells. (A) HNF4α, RXRα, and PPARα expression plasmids were transfected into 293T cells. After 72 h, mRNA expression levels were quantified by qPCR. Control cells were untransfected 293T. ATRA at 1 μmol/L and clofibric acid at 1 mmol/L, were used as ligands to activate the RXRα and PPARα nuclear hormone receptors, respectively. Plasmid pBR322‐HBV1.0 (0.5 μg) was transfected into cells together with or without nuclear hormone receptors (HNF4α 0.2 μg, RXRα 0.2 μg, PPARα 0.2 μg). (B) At 72 h after transfection, HBV cccDNA was quantified by absolute quantitative PCR, and then calculated as copies per cell. (C) HBV pgRNA was detected by RT‐qPCR. (D) HBsAg was detected by time‐resolved fluorescence. (E) HBeAg was detected by ELISA. (F) HBV DNA in the supernatant was extracted and quantified using an HBV DNA real‐time PCR Assay kit. Results are the means ± SD of three repeats, with differences assessed using Student's t test (* P < .05, ** P < .01, *** P < .001)
Article Snippet: The supernatants were harvested and used to determine the concentrations of HBsAg and HBeAg using
Techniques: Expressing, Transfection, Control, Plasmid Preparation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Fluorescence, Enzyme-linked Immunosorbent Assay